DNA

Part:BBa_K1123012

Designed by: Ardjan van der Linden   Group: iGEM13_TU-Eindhoven   (2013-09-08)


His-Tag and Thrombine Cleavage Site

This construct contains the DNA sequence we used to provide a His-Tag and Thrombine cleavage site between our promoter and protein sequence. This will enable us to purify our protein after expression.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None